Π ΠΎΠ»Ρ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Bacillus subtilis
ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΠΌΡΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Π. subtilis, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠΈΠ½ΡΠ΅Π· ΡΡΠ°Π½ΡΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ°, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π·Π° ΡΠΊΡΠΏΠΎΡΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΊΠΈ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π»ΠΈΠ΄Π΅ΡΠ½Π°Ρ ΠΎΠ±Π»Π°ΡΡΡ Π³Π΅Π½Π° pbuE ΠΊΠΎΠ΄ΠΈΡΡΠ΅Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³Π»Π°Π²Π½ΡΠΌΠΈ Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌΠΈ ΡΡΡΠ΅ΠΊΡΠΎΡΠ°ΠΌΠΈ, ΡΡΠΎΠΉ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ ΡΠ²Π»ΡΡΡΡΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΡ* Π°Π΄Π΅Π½ΠΈΠ½ ΠΈ Π΄ΠΈΠ°ΠΌΠΈΠ½ΠΎΠΏΡΡΠΈΠ½, Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ … Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠ»Π°Π²Π° 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. 1. ΠΡΡΠΈΠ½ΠΎΠ²ΡΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ Ρ Bacillus subtilis ΠΈ Π΅Π³ΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ. ΡΡΡ
- 1. 1. 1. Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² de novo. ΡΡΡ
- 1. 1. 2. Π€Π΅ΡΠΌΠ΅Π½ΡΡ, ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΠ΅ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° IMP de novo. ΡΡΡ
- 1. 1. 3. ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΡΠΈΠ½ΠΎΠ² Ρ Π. subtilis. ΡΡΡ
- 1. 1. 4. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Π. subtilis. ΡΡΡ
- 1. 2. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ
Π ΠΠ. ΡΡΡ
- 1. 2. 1. Π‘ΡΡΡΠΊΡΡΡΠ½Π°Ρ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 2. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 3. ΠΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 4. ΠΡΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠ΅Π½ΡΠΎΡΠ½ΡΠ΅ Π ΠΠ. ΡΡΡ
- 1. 2. 4. 1. Π‘ΡΡΡΠΊΡΡΡΠ° Π°ΠΏΡΠ°ΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π΄ΠΎΠΌΠ΅Π½Π° ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 4. 2. ΠΡΠ°Π½ΠΈΠ½ΠΎΠ²Π°Ρ ΠΈ Π°Π΄Π΅Π½ΠΈΠ½ΠΎΠ²Π°Ρ ΡΠ΅Π½ΡΠΎΡΠ½Π°Ρ Π ΠΠ: ΠΏΠΎΡ ΠΎΠΆΠΈΠ΅ Π°ΠΏΡΠ°ΠΌΠ΅ΡΡ, Π½ΠΎ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ Π»ΠΈΠ³Π°Π½Π΄Ρ. ΡΡΡ
- 1. 2. 4. 3. Π Π°Π·Π½ΠΎΠ²ΠΈΠ΄Π½ΠΎΡΡΠΈ 2 '-Π΄Π΅Π·ΠΎΠΊΡΠΈΠ³ΡΠ°Π½ΠΎΠ·ΠΈΠ½-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ
- 1. 1. ΠΡΡΠΈΠ½ΠΎΠ²ΡΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ Ρ Bacillus subtilis ΠΈ Π΅Π³ΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ. ΡΡΡ
- I. Π ΠΠ. ΡΡΡ
- 1. 2. 4. 4. PreQi (7-Π°ΠΌΠΈΠ½ΠΎΠΌΠ΅ΡΠΈΠ»-7-Π΄Π΅Π·Π°Π·Π°Π³ΡΠ°Π½ΠΈΠ½) ΡΠ΅Π½ΡΠΎΡΠ½ΡΠ΅ Π ΠΠ. ΡΡΡ
- 1. 2. 4. 5. ΠΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 4. 5. 1. Π’Π°Π½Π΄Π΅ΠΌΠ½ΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π³ΡΠ°Π½ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ Π°ΠΏΡΠ°ΠΌΠ΅ΡΠ° ΠΈ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ° ykkC. ΡΡΡ
- 1. 2. 4. 5. 2. ΠΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ ΡΠ΅ΡΠΌΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π°ΡΠΏΠ΅ΠΊΡΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΡΡΡ
- 1. 2. 4. 5. 3. ΠΡΠ°Π½ΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠ΅Π½ΡΠΎΡΠ½ΡΠ΅ Π ΠΠ ΠΊΠ°ΠΊ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΌΠΈΡΠ΅Π½ΠΈ. ΡΡΡ
- 1. 3. AICAR ΠΊΠ°ΠΊ ΡΠ΅1ΡΠ»ΡΡΠΎΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°. ΡΡΡ
- 1. 3. 1. ΠΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ AICAR Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ . ΡΡΡ
- 1. 3. 2. Π ΠΎΠ»Ρ AICAR Π² ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ. ΡΡΡ
- 1. 3. 3. Π£ΡΠ°ΡΡΠΈΠ΅ AICAR Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ. ΡΡΡ
- 1. 2. 4. 4. PreQi (7-Π°ΠΌΠΈΠ½ΠΎΠΌΠ΅ΡΠΈΠ»-7-Π΄Π΅Π·Π°Π·Π°Π³ΡΠ°Π½ΠΈΠ½) ΡΠ΅Π½ΡΠΎΡΠ½ΡΠ΅ Π ΠΠ. ΡΡΡ
- 2. 1. ΠΠ°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ ΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ. ΡΡΡ
- 2. 2. Π‘ΡΠ΅Π΄Ρ ΠΈ ΡΡΠ»ΠΎΠ²ΠΈΡ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΡΡΡ
- 2. 3. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΠΠ Π. subtilis. ΡΡΡ
- 2. 4. ΠΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΈ Ρ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ. ΡΡΡ
- 2. 5. ΠΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ². ΡΡΡ
- 2. 6. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ (ΠΠ¦Π ). ΡΡΡ
- 2. 7. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΎΡΠΈΡΡΠΊΠ° ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΠ¦Π .). ΡΡΡ
- 2. 8. Π‘Π°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·. ΡΡΡ
- 2. 9. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΡΡΠ°ΡΠΈΠΉ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 2. 10. ΠΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ ΡΡΡΠ·ΠΎΠ² purE-lacZ. ΡΡΡ
- 2. 11. ΠΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ ΡΡΡΠ·ΠΎΠ²purH-lacZ. ΡΡΡ
- 2. 12. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π΄Π΅Π»Π΅ΡΠΈΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π³/Π³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 2. 13. Π’ΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ in vitro. ΡΡΡ
- 2. 14. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ /3-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π·Ρ. ΡΡΡ
- 2. 15. Π£ΡΠ»ΠΎΠ²ΠΈΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ. ΡΡΡ
- 2. 16. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ AICAR Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ. ΡΡΡ
- 3. 1. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ
ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΠΏΠ΅ΡΠΎΠ½Π° Bacillus subtilis. ΡΡΡ
- 3. 1. 1. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° ΠΈ Π΅Π΅ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ·. ΡΡΡ
- 3. 1. 2. Π‘Π°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π· Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 3. 1. 3. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΡ ΡΡΡΠ·ΠΎΠ² purE-lacZe ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π. subtilis. ΡΡΡ
- 3. 1. 4. ΠΠΏΡΡΡ in vitro ΠΏΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠ² ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 3. 1. 5. Π‘ΠΈΠ½Π΅ΡΠ³ΠΈΠ΄Π½ΡΠΉ ΡΡΡΠ΅ΠΊΡ ΠΌΡΡΠ°ΡΠΈΠΈ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠΌ Π»ΠΎΠΊΡΡΠ΅ purR ΠΈ Π΄Π΅Π»Π΅ΡΠΈΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 3. 1. 6. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ Π²Π½ΡΡΡΠ΅Π½Π½Π΅Π³ΠΎ Rho-Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π΄ΠΈΡΡΠ°Π»ΡΠ½ΡΡ Π³Π΅Π½ΠΎΠ² ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. ΡΡΡ
- 3. 2. ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Bacillus subtilis ΠΈ Π΅Π³ΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ. ΡΡΡ
- 3. 2. 1. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠ°ΡΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Π³Π΅Π½Π° pbuE. ΡΡΡ
- 3. 2. 2. ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½Π° pbuE. ΡΡΡ
- 3. 2. 3. Π‘Π°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π· Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE. ΡΡΡ
- 3. 2. 4. Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΌΡΡΠ°Π½ΡΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ Π·Π°ΠΌΠ΅Π½Ρ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE. ΡΡΡ
3.3. Π Π΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Bacillus subtilis Ρ ΡΠ΅Π»ΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΠ°ΠΌΠΌΠ°-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° AICAR (Π°ΠΊΠ°Π΄Π΅Π·ΠΈΠ½Π°) — ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠ° Π² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ. ΡΡΡ.
3.3.1. Π£ΡΠΈΠ»Π΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. subtilis. ΡΡΡ.
3.3.2. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π΄Π΅Π»Π΅ΡΠΈΠΈ Π³Π΅Π½Π° ΡΠΈΠ³Π Π² ΡΠΎΡΡΠ°Π²Π΅ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΡ Π. subtilis. ΡΡΡ.
3.3.3. ΠΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π²Π΅ΠΊΡΠΎΡΠ° Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pDG268. ΡΡΡ.
3.3.4. Π‘Π°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π· Π³Π΅Π½Π° prs Π. coli (prs%). ΡΡΡ.
3.3.5. Π‘Π°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π· Π³Π΅Π½Π° purF Π. coli (purF^). ΡΡΡ.
3.3.6. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ² Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ
AICAR. ΡΡΡ.
ΠΠ»Π°Π²Π° 4. ΠΠΠ‘Π£ΠΠΠΠΠΠ Π ΠΠΠ£ΠΠ¬Π’ΠΠ’ΠΠ. ΡΡΡ.
ΠΠ«ΠΠΠΠ«. ΡΡΡ.
Π ΠΎΠ»Ρ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Bacillus subtilis (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ ΡΠ΅ΠΌΡ
.
ΠΠ°ΡΡΠΎΡΡΠ°Ρ ΡΠ°Π±ΠΎΡΠ° ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠΎΠ»ΠΈ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Bacillus subtilis. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΎ Π½Π° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΎΡΠΊΡΡΠ²Π°ΡΡΠΈΡ Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠ°ΠΌΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². ΠΠΎΠ½Π΅ΡΠ½ΡΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΡ — Π°Π΄Π΅Π½ΠΎΠ·ΠΈΠ½ΠΌΠΎΠ½ΠΎΡΠΎΡΡΠ°Ρ ΠΈ Π³ΡΠ°Π½ΠΎΠ·ΠΈΠ½ΠΌΠΎΠ½ΠΎΡΠΎΡΡΠ°Ρ (ΠΠΠ ΠΈ GMP), Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΡ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΈ ΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅, ΡΠ²Π»ΡΡΡΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ°ΠΌΠΈ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ ΠΊΠΈΡΠ»ΠΎΡ ΠΈ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΡΠΈΠΊΠ°ΠΌΠΈ, Π½ΠΎ ΠΈ Π²ΡΠΏΠΎΠ»Π½ΡΡΡ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ Π²Π°ΠΆΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ Π³Π»ΠΎΠ±Π°Π»ΡΠ½ΡΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠ² ΠΎΠ±ΡΠ΅Π³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°. Π’ΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΠ΅ ΡΠΏΠΎΡΠΎΠ±Ρ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° ΠΏΡΡΠΈΠ½ΠΎΠ² ΠΎΡΠ½ΠΎΠ²Π°Π½Ρ Π½Π° ΠΈΡ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠΈΠ½ΡΠ΅Π·Π΅, ΠΊΠΎΡΠΎΡΡΠΉ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΡΠ°ΠΌΠΌΠ°ΠΌΠΈ — ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ°ΠΌΠΈ. Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΡ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΡΡ ΡΡΠ°ΠΌΠΌΠΎΠ² ΡΡΠ΅Π±ΡΠ΅Ρ ΡΠ³Π»ΡΠ±Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°, ΡΡΠΎ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π·Π°Π΄Π°Ρ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ·Π±ΡΠ°Π½ Bacillus subtilis, ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌ, ΠΊΠΎΡΠΎΡΡΠΉ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎ ΡΠ»ΡΠΆΠΈΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΌΠΎΠ΄Π΅Π»ΡΡ Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΡΠΈΠ½ΠΎΠ², Π½ΠΎ ΠΈ ΠΎΡΠ½ΠΎΠ²ΠΎΠΉ Π΄Π»Ρ ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠΎΠ².
ΠΡΡΠΈΠ½ΠΎΠ²ΡΠΉ ΠΎΠΏΠ΅ΡΠΎΠ½ Π. subtilis purEKBCSOLFMNHD (Π΄Π°Π»Π΅Π΅ — ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½) ΠΊΠΎΠ΄ΠΈΡΡΠ΅Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ ΡΠΈΠ½ΡΠ΅Π·Π° de novo ΠΈΠ½ΠΎΠ·ΠΈΠ½ΠΌΠΎΠ½ΠΎΡΠΎΡΡΠ°ΡΠ° (IMP), ΠΎΠ±ΡΠ΅Π³ΠΎ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠ° ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² ΠΠΠ ΠΈ GMP. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ Π³Π΅Π½Ρ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Π. subtilis ΠΏΠΎΠ΄Π²Π΅ΡΠΆΠ΅Π½Ρ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΠΈΠ½ΠΈΡΠΈΠ°ΡΠΈΠΈ ΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ. Π ΡΠ»ΡΡΠ°Π΅ Π΄Π²ΡΡ ΠΎΠΏΠ΅ΡΠΎΠ½ΠΎΠ²: ΡΠΈΠ³ΠΎΠΏΠ΅ΡΠΎΠ½Π° ΠΈ xpt-pbuX, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠΈΠ½ΡΠ΅Π· ΠΊΡΠ°Π½ΡΠΈΠ½ΡΠΎΡΡΠΎΡΠΈΠ±ΠΎΠ·ΠΈΠ»ΡΡΠ°Π½ΡΡΠ΅ΡΠ°Π·Ρ, ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΡΡΡ ΠΊΠ°ΠΊ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΠΈΠ½ΠΈΡΠΈΠ°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ Π±Π΅Π»ΠΊΠ°-ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ° PurR, ΡΠ°ΠΊ ΠΈ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ. ΠΠ° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΡΠΈΡ ΠΎΠΏΠ΅ΡΠΎΠ½ΠΎΠ² Π±ΡΠ» ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ Π½Π΅ΠΎΠ±ΡΡΠ½ΡΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡ-Π·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ Π°ΡΡΠ΅Π½ΡΠ°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ: ΠΏΡΠΈ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠΈ Π² ΡΠΎΡΡΠΎΠ²ΡΡ ΡΡΠ΅Π΄Ρ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ Π³ΠΈΠΏΠΎΠΊΡΠ°Π½ΡΠΈΠ½Π° ΠΈ Π³ΡΠ°Π½ΠΈΠ½Π° ΠΈΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΡΠ΅ΡΡΡ ΠΏΠ΅ΡΠ΅Π΄ ΠΏΠ΅ΡΠ²ΡΠΌΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΡΠΌΠΈ Π³Π΅Π½Π°ΠΌΠΈ, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Π² ΠΈΡ ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠ±ΠΈΡΡΠ΅ΡΡΡ ΠΏΠΎΠ»Π½ΡΠΉ ΠΎΠΏΠ΅ΡΠΎΠ½. ΠΠ°ΠΊ Π²ΡΡΡΠ½ΠΈΠ»ΠΎΡΡ ΠΏΠΎΠ·Π΄Π½Π΅Π΅, Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΏΡΡΠΈΠ½ΠΎΠ², Π½Π° ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π³Π΅Π½ΠΎΠ² Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ, ΠΊΠΎΡΠΎΡΡΠΉ Π±ΡΠ» Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ Π² Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π±ΠΈΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ Π³Π΅Π½Π΅ΡΠΈΠΊΠΈ Π€ΠΠ£Π ΠΠΎΡΠΠΠΠ³Π΅Π½Π΅ΡΠΈΠΊΠ° Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠΈΠ±ΠΎΡΠ»Π°Π²ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈ ΡΠΈΠ°ΠΌΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΠΏΠ΅ΡΠΎΠ½ΠΎΠ² Ρ Π. Π·Π½Π«Π¨Π±. Π’Π°ΠΊΠΈΠ΅ ΡΠ΅Π½ΡΠΎΡΠ½ΡΠ΅ Π ΠΠ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠ΅ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΠΌΠ ΠΠ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ ΠΎΠΏΠ΅ΡΠΎΠ½ΠΎΠ², ΠΏΠΎΠ»ΡΡΠΈΠ»ΠΈ Π½Π°ΠΈΠΌΠ΅Π½ΠΎΠ²Π°Π½ΠΈΠ΅ «ΡΠΈΠ±ΠΎΠΏΠ΅ΡΠ΅ΠΊΠ»ΡΡΠ°ΡΠ΅Π»Π΅ΠΉ» (ΠΏΠ¬ΠΎΡΡ ΡΠΉΡΠ¬), ΡΠ°ΠΊ ΠΊΠ°ΠΊ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΡΠΌΠΎΠ³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠΌ ΠΎΠ½ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΌΠΎΠ΄ΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΏΡΠΈΠ»Π΅Π³Π°ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ², Π²ΠΊΠ»ΡΡΠ°Ρ ΠΈΠ»ΠΈ Π²ΡΠΊΠ»ΡΡΠ°Ρ ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΈΠ»ΠΈ ΠΈΠ½ΠΈΡΠΈΠ°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ. β’.
Π Π½Π°ΡΡΠΎΡΡΠ΅ΠΌΡ ΠΌΠΎΠΌΠ΅Π½ΡΡ Π²ΡΡΡΠ½ΠΈΠ»ΠΎΡΡ, ΡΡΠΎ Π»ΠΈΠ΄Π΅ΡΠ½ΡΠ΅ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΡΠ΄Π° Π³Π΅Π½ΠΎΠ² ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ Π. ΠΠΈΠͺΠΠ¨, Π²ΠΊΠ»ΡΡΠ°Ρ ΡΠΌΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½, Ρ Ρ^ΡΠ¬ΠΈΠ₯-ΠΎΠΏΠ΅ΡΠΎΠ½ ΠΈ Π³Π΅Π½ ΡΠ¬ΠΈΠ, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΠΉ ΡΡΠ°Π½ΡΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΠΉ Π±Π΅Π»ΠΎΠΊ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠΉ Π·Π° ΡΡΠ°Π½ΡΠΏΠΎΡΡ, ΠΏΡΡΠΈΠ½ΠΎΠ² ΠΈΠ· ΠΊΠ»Π΅ΡΠΊΠΈ, ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ, ΠΏΠΎΠ»ΡΡΠΈΠ²ΡΡΡ Π½Π°ΠΈΠΌΠ΅Π½ΠΎΠ²Π°Π½ΠΈΠ΅ Π-Π±ΠΎΠΊΡ, ΠΊΠΎΡΠΎΡΠ°Ρ ΡΠΏΠΎΡΠΎΠ±Π½Π° Π²ΡΡΡΡΠΏΠ°ΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠ΅Π½ΡΠΎΡΠ° ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠΉΠ·Π° Π½Π΅ΠΉ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π¨Ρ-Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ. Π' ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ, Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. 8ΠΈΠ¬ΠΠΡ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ΅Π³ΠΎ Π²ΡΡΡΠ½ΠΈΡΡ ΡΠΎΠ»Ρ ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΡΡΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°, Π½Π΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, ΠΎΡΡΠ°Π²Π°Π»Π°ΡΡ Π½Π΅ΡΡΠ½ΠΎΠΉ ΡΠΎΠ»Ρ Π²Π½ΡΡΡΠ΅Π½Π½Π΅Π³ΠΎ ΠΡ-Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ Π² ΠΌΠ΅ΠΆΠ³Π΅Π½Π½ΠΎΠΌ ΡΡΠ°ΡΡΠΊΠ΅ ΡΠΈΠ³Π -ΡΠΈΠ³Π /?ΠΌΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°, Π² ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΠΈΠΈ ΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ².
ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΈΠ³ΠΎΠΏΠ΅ΡΠΎΠ½Π° ΠΈ Π΄ΡΡΠ³ΠΈΡ Π³Π΅Π½ΠΎΠ², Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½Π½ΡΡ Π² ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ ΠΏΡΡΠΈΠ½ΠΎΠ², ΠΈΠΌΠ΅Π΅Ρ ΠΈ Π²Π°ΠΆΠ½ΡΠΉ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°ΡΠΏΠ΅ΠΊΡ, ΠΏΠΎΡΠΊΠΎΠ»ΡΠΊΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΎΡΠ½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, ΠΎΠ±ΡΠ°Π·ΡΡΡΠΈΠ΅ΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΡΠΈΠ½ΠΎΠ², ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΡΠ΄ΠΎΠΌ ΡΡΠ΅Π·Π²ΡΡΠ°ΠΉΠ½ΠΎ Π²Π°ΠΆΠ½ΡΡ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΡΡ ΠΊΠ°ΡΠ΅ΡΡΠ². Π ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, 5-Π°ΠΌΠΈΠ½ΠΎΠΈΠΌΠΈΠ΄Π°Π·ΠΎΠ»-4-ΠΊΠ°ΡΠ±ΠΎΠΊΡΠ°ΠΌΠΈΠ΄ΡΠΈΠ±ΠΎΡΡΡΠ°Π½ΠΎΠ·ΠΈΠ΄ ΠΈΠ»ΠΈ ΡΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΠΎ — AICAR (ΡΠ°ΠΊΠΆΠ΅ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠΉ ΠΊΠ°ΠΊ Π°ΠΊΠ°Π΄Π΅Π·ΠΈΠ½), ΠΊΠΎΡΠΎΡΡΠΉ ΠΎΠ±ΡΠ°Π·ΡΠ΅ΡΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΡΠΈΠ½ΠΎΠ² de novo Ρ Π. subtilis, ΡΠ²Π»ΡΠ΅ΡΡΡ Π°ΠΊΡΠΈΠ²Π°ΡΠΎΡΠΎΠΌ Π°Π΄Π΅Π½ΠΎΠ·ΠΈΠ½ΠΌΠΎΠ½ΠΎΡΠΎΡΡΠ°Ρ-Π°ΠΊΡΠΈΠ²ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΊΠΈΠ½Π°Π·Ρ (ΠΠΠ Π), ΠΊΠΎΡΠΎΡΠ°Ρ, Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ, ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π»ΠΎΠ±Π°Π»ΡΠ½ΡΠΌ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠΌ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ², ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΡ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΠ°ΡΡΡ ΡΡΠΊΠ°ΡΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡΡ ΡΡΠ΄ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π°ΠΊΠ°Π΄Π΅Π·ΠΈΠ½Π°, ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΊΠΎΡΠΎΡΡΡ Π΄Π°ΡΡ ΠΎΠ±Π½Π°Π΄Π΅ΠΆΠΈΠ²Π°ΡΡΠΈΠ΅ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅, Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π±ΠΎΡΡΠ±Ρ Ρ. Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π»Π΅ΠΉΠΊΠ΅ΠΌΠΈΠ΅ΠΉ Π-ΠΊΠ»Π΅ΡΠΎΠΊ, Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° ΠΈ ΠΊΠ°ΡΠ΄ΠΈΠΎΡ ΠΈΡΡΡΠ³ΠΈΠΈ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ, ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ°-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° AICAR Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠ΅ΠΉ Π³Π΅Π½ΠΎΠ² ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ Π²Π΅ΡΡΠΌΠ° Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ Π΄Π»Ρ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π΄Π΅ΡΠ΅Π²ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½ΡΠ΅Π·Π° AICAR.
Π¦Π΅Π»Ρ ΠΈ Π·Π°Π΄Π°ΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
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Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠ° ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° ΠΈ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠ° ΠΏΡΡΠΈΠ½ΠΎΠ² Ρ Π. subtilis Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ* ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ ΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π½Π° ΡΡΠΎΠΉ ΠΎΡΠ½ΠΎΠ²Π΅ ΡΡΠ°ΠΌΠΌΠ°-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° AICAR — ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠ° Π² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ.
Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΈ ΡΠ΅ΡΠ΅Π½Ρ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ Π·Π°Π΄Π°ΡΠΈ:
1. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ /?ΠΌΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. subtilis ΠΈ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»Π΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠΎΠ³ΠΎ ΠΎΠΏΠ΅ΡΠΎΠ½Π°.
2. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Π. subtilis, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠΈΠ½ΡΠ΅Π· ΡΡΠ°Π½ΡΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ°, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π·Π° ΡΠΊΡΠΏΠΎΡΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠ², Π²Π»ΠΈΡΡΡΠΈΡ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½Π° pbuE.
3. Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΡΠ°ΠΌΠΌΠ°-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ°, Π½Π°ΠΊΠ°ΠΏΠ»ΠΈΠ²Π°ΡΡΠ΅Π³ΠΎ AICAR Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ Π² ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΡ , Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ Π΄Π»Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅Π³ΠΎ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π°.
ΠΠ°ΡΡΠ½Π°Ρ Π½ΠΎΠ²ΠΈΠ·Π½Π° ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅Π½Π½ΠΎΡΡΡ ΡΠ°Π±ΠΎΡΡ.
Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ" Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ /Π·ΠΌΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. subtilis, Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠΎΠ³ΠΎ ΠΎΠΏΠ΅ΡΠΎΠ½Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΅Π΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ°ΠΌΠΈ-ΡΡΡΠ΅ΠΊΡΠΎΡΠ°ΠΌΠΈ Π³ΡΠ°Π½ΠΈΠ½ΠΎΠΌ ΠΈ Π³ΠΈΠΏΠΎΠΊΡΠ°Π½ΡΠΈΠ½ΠΎΠΌ. ΠΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ΅ΡΡΡΠ΅Ρ Π΄ΠΈΡΡΠ°Π»ΡΠ½ΡΡ Π³Π΅Π½ΠΎΠ² pwr-ΠΎΠΏΠ΅ΡΠΎΠ½Π° (purMNHD) ΠΏΠΎΠ΄Π°Π²Π»ΡΠ΅ΡΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ Π² 2 ΡΠ°Π·Π° Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Rho-Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠ° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ Π² ΠΌΠ΅ΠΆΠ³Π΅Π½Π½ΠΎΠΌ ΡΡΠ°ΡΡΠΊΠ΅ purF-purM.
ΠΠ° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΡΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Π. subtilis, Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ Π² ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π»ΠΈΠ΄Π΅ΡΠ° (A-Π±ΠΎΠΊΡ) Π΄Π²ΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ Π·Π°ΠΌΠ΅Π½ 70U—"Π‘ ΠΈ Π100—"G ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ in vivo: Π²ΠΌΠ΅ΡΡΠΎ Π°Π΄Π΅Π½ΠΈΠ½Π° ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΡΠΌ ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠΌ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡΡ Π³ΡΠ°Π½ΠΈΠ½.
ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΡ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. subtilis ΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Π. subtilis Ρ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠΌΠΈ Π·Π°ΠΌΠ΅Π½Π°ΠΌΠΈ Π² ΡΡΠ°ΡΡΠΊΠ°Ρ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΌΠ ΠΠ, ΠΊΠΎΡΠΎΡΡΠ΅ Π²Π»ΠΈΡΡΡ Π½Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΡΠΎΡΠΈΡΠ½ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ ΠΈΠ»ΠΈ Π½Π° ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΠΌΠ ΠΠ Ρ ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠΌ. ΠΠΎΠ»Π»Π΅ΠΊΡΠΈΡ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° Π΄Π»Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π² ΡΡΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π²ΡΡΠΎΠΊΠΎΠ°ΠΊΡΠΈΠ²Π½ΡΡ ΡΡΠ°ΠΌΠΌΠΎΠ²-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠΎΠ².
Π‘ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ Π²ΡΡΠΎΠΊΠΎΠ°ΠΊΡΠΈΠ²Π½ΡΠΉ ΡΡΠ°ΠΌΠΌ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½Ρ, Π½Π°ΠΊΠ°ΠΏΠ»ΠΈΠ²Π°ΡΡΠΈΠΉ 1113 Π³/Π» AICAR Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ ΡΡΠ°ΠΌΠΌ ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΡΠ»ΡΠΆΠΈΡΡ ΠΎΡΠ½ΠΎΠ²ΠΎΠΉ Π΄Π»Ρ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½ΡΠ΅Π·Π° AICAR — ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠ° Π² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ.
ΠΠ«ΠΠΠΠ«:
1. ΠΡΡΠ²Π»Π΅Π½Π° ΡΠΎΠ»Ρ Π΄Π²ΡΡ Rho-Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΡΡ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠΎΠ² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ, ΠΎΠ΄ΠΈΠ½ ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ Π² Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ, Π° Π²ΡΠΎΡΠΎΠΉ — Π² ΠΌΠ΅ΠΆΠ³Π΅Π½Π½ΠΎΠΌ ΡΡΠ°ΡΡΠΊΠ΅ purF-purM, Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠΌΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½Π°Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ /?"Π³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΠΏΡΠΈ Π΄Π΅Π»Π΅ΡΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΎΠ±ΠΎΠΈΡ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΎΡΠΎΠ² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π° purR.
2. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ°ΠΉΡ-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π° ΠΈΠ·ΡΡΠ΅Π½Π° ΡΡΡΡΠΊΡΡΡΠ½ΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ, ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΈΠ³-ΠΎΠΏΠ΅ΡΠΎΠ½Π° Π. subtilis. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΏΠΎΠΌΠΈΠΌΠΎ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ Π±Π΅Π»ΠΊΠ°-ΡΠ΅ΠΏΡΠ΅ΡΡΠΎΡΠ° PurR, ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠΎΠ³ΠΎ ΠΎΠΏΠ΅ΡΠΎΠ½Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΅Π΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ' ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ°ΠΌΠΈ-ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠΌΠΈ Π³ΡΠ°Π½ΠΈΠ½ΠΎΠΌ ΠΈ Π³ΠΈΠΏΠΎΠΊΡΠ°Π½ΡΠΈΠ½ΠΎΠΌ.
3. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΠΌΡΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° pbuE Π. subtilis, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠΈΠ½ΡΠ΅Π· ΡΡΠ°Π½ΡΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ°, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π·Π° ΡΠΊΡΠΏΠΎΡΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΊΠΈ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π»ΠΈΠ΄Π΅ΡΠ½Π°Ρ ΠΎΠ±Π»Π°ΡΡΡ Π³Π΅Π½Π° pbuE ΠΊΠΎΠ΄ΠΈΡΡΠ΅Ρ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΡΡ ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ Π ΠΠ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³Π»Π°Π²Π½ΡΠΌΠΈ Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌΠΈ ΡΡΡΠ΅ΠΊΡΠΎΡΠ°ΠΌΠΈ, ΡΡΠΎΠΉ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ ΡΠ²Π»ΡΡΡΡΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΡ* Π°Π΄Π΅Π½ΠΈΠ½ ΠΈ Π΄ΠΈΠ°ΠΌΠΈΠ½ΠΎΠΏΡΡΠΈΠ½, Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ Π² ΡΡΠ΅Π΄Ρ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΡΠΈΠ»Π΅Π½ΠΈΡΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½Π° pbuE.
4. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ Π² ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π»ΠΈΠ΄Π΅ΡΠ½ΠΎΠΉ Π ΠΠ Π³Π΅Π½Π° pbuE (A-Π±ΠΎΠΊΡ) Π΄Π²ΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ Π·Π°ΠΌΠ΅Π½ 70U—"Π‘ ΠΈ Π100—*G ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π½ΡΠΎΡΠ½ΠΎΠΉ Π ΠΠ in vivo: Π²ΠΌΠ΅ΡΡΠΎ Π°Π΄Π΅Π½ΠΈΠ½Π° ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΡΠΌ ΡΡΡΠ΅ΠΊΡΠΎΡΠΎΠΌ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡΡ Π³ΡΠ°Π½ΠΈΠ½. ΠΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° ΡΡΠ΅Ρ ΠΌΠ΅ΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ A-Π±ΠΎΠΊΡΠ° Π³Π΅Π½Π° pbuE Π. subtilis.
5. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ ΠΏΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Ρ ΡΡΠ°ΠΌΠΌΠ° Π. subtilis, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½Π°Ρ Π½Π° ΡΠ²Π΅ΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΡ AICAR ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ.
ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΌΠ°Π½ΠΈΠΏΡΠ»ΡΡΠΈΠΉ ΡΡΠ°ΠΌΠΌ Π. Π±ΡΠ«Π¨Π± Π½Π°ΠΊΠ°ΠΏΠ»ΠΈΠ²Π°Π΅Ρ 11−13 Π³/Π» ΠΠΠ‘ΠΠ Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ.
Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
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