Функции цистеин-богатых белков гордеивирусов

выводы.

1. Показано, что белок уЬ ПЛВМ, как и белок уЬ ВШМЯ, осуществляет регуляцию экспрессии вирусных БО и ТБГ1, а также синтеза вирусных РНК.

2. Установлено, что белок уЬ ВШМЯ является фактором дальнего транспорта в N. ЬеШкатгапа и по-разному осуществляет регуляцию экспрессии вирусных БО и ТБГ1 в однодольном и двудольном растениях-хозяевах.

3. Показана способность белка уЬ ПЛВМ усиливать проявление симптомов близкородственного вируса ВШМЯ, а также способность белков уЬ ПЛВМ и ВШМЯ увеличивать патогенность неродственного Х-вируса картофеля.

4. Доказана способность цистеин-богатых белков уЬ гордеивирусов ВШМЯ и ПЛВМ подавлять посттранскрипционное умолкание генов.

5. Методом сайт-направленного мутагенеза картированы последовательности белка уЬ ПЛВМ, необходимые для супрессии посттранскрипционного умолкания генов.

БЛАГОДАРНОСТИ.

В заключение хочу выразить искреннюю благодарность Сергею Юрьевичу Морозову и Андрею Геннадьевичу Соловьеву — моим первым учителям и научным руководителям за неоценимую помощь, поддержку и внимание. Хочу также поблагодарить заведующего кафедрой вирусологии, академика Атабекова Иосифа Григорьевича за предоставленную возможность выполнить данную работу. Выражаю признательность Евгению Ивановичу Савенкову, Наталии Олеговне Калининой, Татьяне Николаевне Ерохиной, Виктору Константиновичу Новикову, Андрею Геннадьевичу Клюшину, Светлане Дмитриевне Зверевой, Дарье Викторовне Ракитиной, Нине Иосифовне Луховицкой, Андрею Александровичу Замятнину, Марине Владимировне Архипенко и Герхильд Юттнер за оказанную помощью в работе. Благодарю также наших иностранных коллег проф. Болкомба, проф. Валконена, проф. Джексона, д-ра Меритса, д-ра Шимана за предоставленные материалы. Большое спасибо моим преподавателям и всем сотрудникам кафедры вирусологии, которые помогали мне все эти годы.

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254. РОССИЙСКАЯ ГОСУДАРСТВЕННАЯ БИБЛИОТЕКАо¿-0945−2 б.