ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ Π΅Π΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΈΠΌΠΏΠΎΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
Π Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ ΠΈΠ·ΡΡΠ°ΡΡΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ. Π ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Saccharomyces cerevisiae Π΅Π΄ΠΈΠ½ΡΡΠ²Π½Π½Π°Ρ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²Π°Ρ ΡΠ ΠΠ Ρ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½ΠΎΠΌ CUU (ΡΠ ΠΠ!) ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΡΡΠ³Π°Ρ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²Π°Ρ ΡΠ ΠΠ Ρ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½ΠΎΠΌ UUU (ΡΠ ΠΠ2) ΠΏΡΠΈΡΡΡΡΡΠ²ΡΠ΅Ρ ΡΠΎΠ»ΡΠΊΠΎ Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅. Π₯ΠΎΡΡ ΡΡΡΠ΅ΡΡΠ²ΡΡΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΡΠ ΠΠ1 ΠΈ ΡΠ ΠΠ2, ΠΎΠ±Π΅ ΠΎΠ½ΠΈ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΡΡΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈ ΡΠΎΠΉ ΠΆΠ΅… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΠΠ‘ΠΠ Π‘ΠΠΠ ΠΠ©ΠΠΠΠ
- ΠΠΠΠΠΠΠΠ
- ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. 1. ΠΠΌΠΏΠΎΡΡ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ
- 1. 1. 1. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ
- 1. 1. 2. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ
- 1. 1. 3. ΠΠΌΠΏΠΎΡΡ Π ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
- 1. 1. 4. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΠ°ΡΡΠΊΠ°Π³ΠΎΡΡΡΠ³Ρ ΡΠ΅Π³Π΅ΡΠ³81Π°Π΅
- 1. 2. ΠΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌ ΠΈΠΌΠΏΠΎΡΡΠ° Π ΠΠ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ 21 1.2.1 Π£ΡΠ°ΡΡΠΈΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 1. 2. 2. Π£ΡΠ°ΡΡΠΈΠ΅ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 1. 2. 2. 1. ΠΠ·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΡΠ ΠΠ Ρ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·Π°ΠΌΠΈ
- 1. 2. 2. 2. Π£ΡΠ°ΡΡΠΈΠ΅ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π· Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 1. 2. 3. Π‘ΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΡΠ ΠΠ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠ΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ°
- 1. 2. 4. ΠΠ΅Ρ Π°Π½ΠΈΠ·ΠΌ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ° ΡΠ ΠΠ ΡΠ΅ΡΠ΅Π· ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ
- 1. 2. 2. Π£ΡΠ°ΡΡΠΈΠ΅ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 1. 3. Π²ΠΠ¬ΠΠ₯. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Π° Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ
ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌΠΈ ΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ
- 1. 3. 1. ΠΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΡΠΈΠ½ΡΠΈΠΏΡ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΠΠ¬ΠΠ₯
- 1. 3. 2. ΠΠΎΠΈΡΠΊ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΡΠ ΠΠ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ ΠΈΠ·Π±ΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠ΅ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ ΠΠ Π‘Π°Π·Π°ΠΌΠΈ, Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄Π° 8ΠΠ¬ΠΠ₯
- 1. 1. ΠΠΌΠΏΠΎΡΡ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ
- 2. 1. Π¨ΡΠ°ΠΌΠΌΡ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ²
- 2. 2. ΠΠ΅Π½Π½ΠΎΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ
- 2. 2. 1. ΠΡΡ ΠΎΠ΄Π½ΡΠ΅ Π³Π΅Π½Π½ΠΎΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΡΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ
- 2. 2. 2. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·
- 2. 2. 3. ΠΡΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°ΡΠΈΠ»-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ ΡΠ°Π³ΠΎΠ²
- 2. 2. 4. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΠΎΠΉ ΠΠΠ ΠΈΠ· ΡΠ°Π³ΠΎΠ²ΡΡ ΡΠ°ΡΡΠΈΡ 60 2.2.5.ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄ 61 2.2.6. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΎΡΠΈΡΡΠΊΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄
- 2. 3. Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ
- 2. 3. 1. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠ¦Π Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΠΠ-ΠΌΠ°ΡΡΠΈΡ Π΄Π»Ρ Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ
- 2. 3. 2. Π Π΅Π°ΠΊΡΠΈΡ Π’7-ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ in vitro
- 2. 4. ΠΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π· Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ ΠΊΠΈΡΠ»ΠΎΡ Π² ΠΠΠΠ ΠΈ Π² Π°Π³Π°ΡΠΎΠ·Π½ΠΎΠΌ Π³Π΅Π»Π΅
- 2. 5. Π Π°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΌΠ΅ΡΠ΅Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ²
- 2. 6. Π’ΡΠ°Π½ΡΠΏΠΎΡΡ ΡΠ°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎ ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Π³Π΅Π½Π° ΡΠ ΠΠ ΠΈ ΡΠ ΠΠ2 Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 2. 7. ΠΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ²
- 2. 8. Π‘Π²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠ² Ρ npe-MSK
- 2. 9. ΠΠΌΠΏΠΎΡΡ Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΡΠ ΠΠ2 Π² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ in vivo
- 2. 9. 1. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ S. cerevisiae
- 2. 9. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΠ ΠΠ S. cerevisiae
- 2. 9. 3. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ S. cerevisiae
- 2. 9. 4. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ ΠΠ
- 2. 9. 5. ΠΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ Ρ ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠΌΠΈ Π·ΠΎΠ½Π΄Π°ΠΌΠΈ
- 2. 10. ΠΠ΅ΡΠΎΠ΄ SELEX
- 2. 10. 1. Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π²ΡΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΠΉ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ Π΄Π»Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ SELEXa
- 2. 10. 2. Π£ΡΠ»ΠΎΠ²ΠΈΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Ρ npe-MSK ΠΈ ΠΈΠΌΠΏΠΎΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 2. 10. 3. ΠΠ±ΡΠ°ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΡ ΠΈ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ
- 2. 10. 4. ΠΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 2. 10. 5. ΠΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΈ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ
- 3. 1. ΠΠ»Π΅ΠΌΠ΅Π½ΡΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠ΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ° Π² Π°ΠΌΠΈΠΈΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠΌ ΡΡΠ΅Π±Π»Π΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ
- 3. 1. 1. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΡΠ ΠΠ! ΠΈ ΡΠ ΠΠ2 in vitro
- 3. 1. 2. ΠΠΌΠΏΠΎΡΡ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ ΡΠ ΠΠ in vivo
- 3. 1. 3. Π ΠΎΠ»Ρ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΉ Π°ΠΌΠΈΠ½ΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ΅Π±Π»Ρ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΌ ΠΈΠΌΠΏΠΎΡΡΠ΅ ΡΠ ΠΠ
- 3. 2. ΠΠΎΠΈΡΠΊ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π² ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ, ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΡΡΠΈΡ ΠΈΠ·Π±ΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΠΈΠΌΠΏΠΎΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ, Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄Π° SELEX
- 3. 2. 1. Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊ Π ΠΠ, ΠΎΡΠΎΠ±ΡΠ°Π½Π½ΡΡ Π² Ρ ΠΎΠ΄Π΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ ΠΏΠΎ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΊ ΠΈΠΌΠΏΠΎΡΡΡ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ
- 3. 2. 2. Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊ Π ΠΠ, ΠΎΡΠΎΠ±ΡΠ°Π½Π½ΡΡ ΠΏΠΎ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΡΡ Ρ npe-MSK
- 3. 2. 3. ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ Π² Ρ ΠΎΠ΄Π΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ
- 3. 2. 4. ΠΠ½Π°Π»ΠΈΠ· ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ²
- 3. 2. 5. ΠΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ
ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ Π΅Π΅ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΈΠΌΠΏΠΎΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΈΠΌΠ΅ΡΡ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΠΉ Π³Π΅Π½ΠΎΠΌ ΠΈ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΠΉ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΎΠ½Π½ΡΠΉ Π°ΠΏΠΏΠ°ΡΠ°Ρ. ΠΠ΄Π½Π°ΠΊΠΎ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π·Π°ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½Ρ Π² ΡΠ΄Π΅ΡΠ½ΠΎΠΌ Π³Π΅Π½ΠΎΠΌΠ΅, ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΡΡΡΡ Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅ ΠΈ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠΈΡΡΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎ Π½Π΅Π΄Π°Π²Π½ΠΎ Π±ΡΠ» ΠΎΡΠΊΡΡΡ ΡΠ°ΠΊΠΆΠ΅ ΠΈΠΌΠΏΠΎΡΡ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ ΠΊΠΈΡΠ»ΠΎΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ [Suyama, 1967; Suyama and Hamada, 1976; Schneider, 1994]. ΠΠΌΠΏΠΎΡΡ ΡΠ ΠΠ Π±ΡΠ» ΠΏΠΎΠΊΠ°Π·Π°Π½ Π΄Π»Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ [Martin et al., 1979], ΠΏΡΠΎΡΡΠ΅ΠΉΡΠΈΡ [Suyama, 1982] ΠΈ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ [Dietrich et al., 1993]. Π ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΡΡΡΡ Π΄ΡΡΠ³ΠΈΠ΅ Π²ΠΈΠ΄Ρ Π ΠΠ, ΠΊΠΎΡΠΎΡΡΠ΅ Π²Ρ ΠΎΠ΄ΡΡ Π² ΡΠΎΡΡΠ°Π² Π ΠΠΠ°Π·Ρ Π ΠΈ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ°ΠΉΡ-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½Π΄ΠΎΡΠΈΠ±ΠΎΠ½ΡΠΊΠ»Π΅Π°Π·Ρ (MRP), Π° ΡΠ°ΠΊΠΆΠ΅ 5S ΡΠ ΠΠ [Li al., 1994; Schneider, 1994]. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΡΡ Π²ΠΈΠ΄ΠΎΠ² ΡΠ ΠΠ ΠΊΠΎΠ»Π΅Π±Π»Π΅ΡΡΡ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². Π£ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ ΡΠΎΠ»ΡΠΊΠΎ ΠΎΠ΄Π½Π° ΡΠ ΠΠ, Π° Ρ ΡΡΠΈΠΏΠ°Π½ΠΎΡΠΎΠΌΠ°ΡΠΈΠ΄ Π²ΡΠ΅ ΡΠ ΠΠ Π·Π°ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½Ρ Π² ΡΠ΄ΡΠ΅. ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠ°ΡΡΡΡΠ΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ° Π ΠΠ, ΠΎΡΠ΅Π½Ρ ΠΌΠ°Π»ΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎ ΠΎ ΡΠΎΠΌ, ΠΊΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ.
ΠΠ΅Π΄Π°Π²Π½ΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ Π² ΡΠ°ΠΌΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ ΠΠ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ° ΠΌΠΎΠ»Π΅ΠΊΡΠ». ΠΠΎΠ΄ΠΎΠ±Π½ΡΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ Π² Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π΅ Π³Π»ΡΡΠ°ΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Tetrahymena pyriformis ΠΈ Π² D-ΠΏΠ΅ΡΠ»Π΅ ΠΈΠ·ΠΎΠ»Π΅ΠΉΡΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Leishmania tarentolae [Rusconi and Cech, 1996bMahapatra et al., 1998].
Π Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ ΠΈΠ·ΡΡΠ°ΡΡΡΡ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ. Π ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ Saccharomyces cerevisiae Π΅Π΄ΠΈΠ½ΡΡΠ²Π½Π½Π°Ρ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²Π°Ρ ΡΠ ΠΠ Ρ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½ΠΎΠΌ CUU (ΡΠ ΠΠ!) ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΡΡΠ³Π°Ρ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²Π°Ρ ΡΠ ΠΠ Ρ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½ΠΎΠΌ UUU (ΡΠ ΠΠ2) ΠΏΡΠΈΡΡΡΡΡΠ²ΡΠ΅Ρ ΡΠΎΠ»ΡΠΊΠΎ Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅. Π₯ΠΎΡΡ ΡΡΡΠ΅ΡΡΠ²ΡΡΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΡΠ ΠΠ1 ΠΈ ΡΠ ΠΠ2, ΠΎΠ±Π΅ ΠΎΠ½ΠΈ Π°ΠΌΠΈΠ½ΠΎΠ°ΡΠΈΠ»ΠΈΡΡΡΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈ ΡΠΎΠΉ ΠΆΠ΅ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠ·ΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·ΠΎΠΉ, KRS. Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΈΠΌΠΏΠΎΡΡΠ° ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π΅Ρ ΡΡΠ°ΡΡΠΈΠ΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π»ΠΈΠ·ΠΈΠ»-ΡΠ ΠΠ-ΡΠΈΠ½ΡΠ΅ΡΠ°Π·Ρ, ΠΏΡΠ΅-MSK [Tarassov et al., 1995Π°]. Π Π°Π½Π΅Π΅ Π² Π½Π°ΡΠ΅ΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ Π² 34 ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π° ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ° [Entelis et al., 1998].
Π¦Π΅Π»Ρ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ — ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΈ ΠΏΠΎΠΈΡΠΊ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΡ Π΅Π΅ ΠΈΠ·Π±ΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠΉ ΠΈΠΌΠΏΠΎΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΈΠΌΠΏΠΎΡΡΠ° Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ ΠΊΠΈΡΠ»ΠΎΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠ²ΡΠ·Π°Π½Ρ Ρ ΠΌΡΡΠ°ΡΠΈΡΠΌΠΈ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΠ ΠΠ. ΠΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΈΠΌΠΏΠΎΡΡΠ° ΡΠ ΠΠ ΠΏΠΎΠΌΠΎΠΆΠ΅Ρ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ ΡΡΠ°Π½ΡΠΏΠΎΡΡΠΈΡΠΎΠ²ΠΊΠΈ Π³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π½Π΅ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²Π΅Π½Π½ΠΎ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ.
ΠΠ«ΠΠΠΠ«.
1. ΠΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ Π°ΠΌΠΈΠ½ΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ΅Π±Π»Ρ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΠΌΠΈ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠΌΠΏΠΎΡΡΠ° Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ1, ΡΠ²Π»ΡΡΡΡΡ ΠΏΠ΅ΡΠ²Π°Ρ ΠΏΠ°ΡΠ° Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ² Π² Π°ΠΌΠΈΠ½ΠΎΠ°ΠΊΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠΌ ΡΡΠ΅Π±Π»Π΅ G1-C72 ΠΈ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄-Π΄ΠΈΡΠΊΡΠΈΠΌΠΈΠ½Π°ΡΠΎΡ U73.
2.
ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅
Π΄Π΅ΡΠ΅ΡΠΌΠΈΠ½Π°Π½Ρ ΠΈΠΌΠΏΠΎΡΡΠ° Π² ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π½Π΅ΠΈΠΌΠΏΠΎΡΡΠΈΡΡΠ΅ΠΌΠΎΠΉ Π»ΠΈΠ·ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΠ ΠΠ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π΅ ΠΈΠΌΠΏΠΎΡΡΠΈΡΠΎΠ²Π°ΡΡΡΡ Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ in vivo.
3. Π Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΌΡ ΠΈΠΌΠΏΠΎΡΡΡ Π² ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ ΡΠ ΠΠ1, ΡΠΎΡ ΡΠ°Π½ΡΡΡΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ D-Π²Π΅ΡΠ²ΠΈ.
4. Π ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ ΡΠΊΠΎΡΠΎΡΠ΅Π½Π½ΡΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΡΠ ΠΠ (50−60 Π½.), ΠΈΠΌΠ΅ΡΡΠΈΠ΅ Π΄Π΅Π»Π΅ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π°, V-ΠΏΠ΅ΡΠ»ΠΈ ΠΈ Π’-Π²Π΅ΡΠ²ΠΈ, Π½Π΅ ΠΈΠΌΠ΅ΡΡΠΈΠ΅ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΡΡ Π²ΡΠΎΡΠΈΡΠ½ΡΡ ΡΡΡΡΠΊΡΡΡΡ ΡΠΈΠΏΠ° «ΠΊΠ»Π΅Π²Π΅ΡΠ½ΠΎΠ³ΠΎ Π»ΠΈΡΡΠ°» .
5. Π‘Π΅Π»Π΅ΠΊΡΠΈΠΈ Π² ΡΠ°ΠΉΠΎΠ½Π΅ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΠΎΠ³ΠΎ Π°Π½ΡΠΈΠΊΠΎΠ΄ΠΎΠ½Π° ΡΠ ΠΠ Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ, ΠΈ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² ΡΡΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π½Π΅ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡΡΡ Π½Π° ΠΈΠ·Π±ΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΈΠΌΠΏΠΎΡΡΠ° Π² ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΈ in vitro.
Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
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