ΠΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠ°Ρ ΠΈ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π΄ΡΠ ΠΠ Π² ΠΊΡΠ»ΡΡΡΡΠ°Ρ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ
Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΏΠ΅ΡΠ²ΠΎΠΎΡΠ΅ΡΠ΅Π΄Π½ΡΡ Π·Π°Π΄Π°Ρ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ, ΠΊΠ°ΠΊ ΠΏΡΡΠ΅ΠΌ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π½ΠΎΠ²ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π»Π΅ΡΠ΅Π½ΠΈΡ, ΡΠ°ΠΊ ΠΈ ΠΏΡΡΠ΅ΠΌ ΡΠΎΠ²Π΅ΡΡΠ΅Π½ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΡ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ΄Ρ ΠΎΠ΄ΠΎΠ². Π₯ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²ΠΎ, ΡΠ°Π΄ΠΈΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΠ΅ ΠΈ Ρ ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΎΡΡΠ°ΡΡΡΡ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. Π ΡΠΎΠΆΠ°Π»Π΅Π½ΠΈΡ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- Π‘ΠΏΠΈΡΠΎΠΊ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠΉ
ΠΠ»Π°Π²Π° 1. ΠΠΎΡΡΠ΅ΠΊΡΠΈΡ Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ siPHK (ΠΎΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ).
1.1. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅: Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΠΈ Π΅Π΅ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΊΠ°ΠΊ ΠΌΠΈΡΠ΅Π½ΠΈ Π΄Π»Ρ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΡ Π ΠΠ.
1.2. Π Π΅Π³ΡΠ»ΡΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°.
1.2.1. Π¦ΠΈΠΊΠ»ΠΈΠ½Ρ ΠΈ ΡΠΈΠΊΠ»ΠΈΠ½-Π·Π°Π²ΠΈΡΠΈΠΌΡΠ΅ ΠΊΠΈΠ½Π°Π·Ρ.
1.2.2. Π Π΅Π³ΡΠ»ΡΡΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΠΊΠ»ΠΈΠ½ΠΎΠ² ΠΈ ΡΠΈΠΊΠ»ΠΈΠ½-Π·Π°Π²ΠΈΡΠΈΠΌΡΡ ΠΊΠΈΠ½Π°Π·.
1.2.3. «Π’ΠΎΡΠΊΠΈ ΠΏΡΠΎΠ²Π΅ΡΠΊΠΈ» ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°.
1.3. ΠΠ°ΡΡΡΠ΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΠΈ ΠΈΡ ΠΏΡΠΈΡΠΈΠ½Ρ.
1.3.1. Her2 (c-erb-B2/Neu).
1.3.2. Π¦ΠΈΠΊΠ»ΠΈΠ½ Π1.
1.3.3. ΠΡΠΎΡΠ΅ΠΈΠ½ΠΊΠΈΠ½Π°Π·Π° Π‘ (Π ΠΠ‘).
1.4. ΠΠ»Π΅ΡΠΎΡΠ½Π°Ρ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠ°.
1.4.1. ΠΡΠΎΡΠ΅ΡΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ.
1.4.2. ΠΠ½Π΄ΡΠΊΡΠΎΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ.
1.5. ΠΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ siPHK.
1.5.1. Π ΠΠ — ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΡ.
1.5.2. ΠΠΊΡΠΈΠ²Π°ΡΠΈΡ ΡΠΈΡΡΠ΅ΠΌΡ Π²ΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π΄ΡΠ ΠΠ.
1.5.3. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΠΏΡΡΠ°Π½ΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ siPHK.
1.5.4. ΠΠ½Π΄ΡΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ siPHK.
ΠΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠ°Ρ ΠΈ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π΄ΡΠ ΠΠ Π² ΠΊΡΠ»ΡΡΡΡΠ°Ρ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΏΠ΅ΡΠ²ΠΎΠΎΡΠ΅ΡΠ΅Π΄Π½ΡΡ Π·Π°Π΄Π°Ρ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ, ΠΊΠ°ΠΊ ΠΏΡΡΠ΅ΠΌ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π½ΠΎΠ²ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π»Π΅ΡΠ΅Π½ΠΈΡ, ΡΠ°ΠΊ ΠΈ ΠΏΡΡΠ΅ΠΌ ΡΠΎΠ²Π΅ΡΡΠ΅Π½ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΡ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠ΄Ρ ΠΎΠ΄ΠΎΠ². Π₯ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²ΠΎ, ΡΠ°Π΄ΠΈΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΠ΅ ΠΈ Ρ ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΎΡΡΠ°ΡΡΡΡ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. Π ΡΠΎΠΆΠ°Π»Π΅Π½ΠΈΡ, Π΄Π°ΠΆΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΌΠ΅ΡΠΎΠΏΡΠΈΡΡΠΈΠΉ, ΡΠ°ΡΡΠΎ Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΏΠΎΠ»Π½ΠΎΠΉ ΡΠ»ΠΈΠΌΠΈΠ½Π°ΡΠΈΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠΎΡΡΠΎΠΌΡ, Π½Π΅ΡΠΌΠΎΡΡΡ Π½Π° Π·Π°ΠΌΠ΅ΡΠ½ΡΠ΅ Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠΎΠΊΡΠΈΡΠ½ΠΎΡΡΠΈ Ρ ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΡΡΠ΅ΠΌ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΠ΅Π΄ΡΡΠ² Π³Π΅Π½-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ²Π»ΡΡΡΡΡ ΠΈΡΠΊΠ»ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌΠΈ.
ΠΠ°ΠΆΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ³ΡΠ°ΡΡ Π³Π΅Π½Ρ, Π³ΠΈΠΏΠ΅ΡΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΊΠΎΡΠΎΡΡΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π½Π΅ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ (Π±Π΅Π»ΠΊΠΈ-ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°) [ 1 ], Π±Π»ΠΎΠΊΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° [ 2 ], Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ [3], ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΠΎΠ²Π½Ρ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ [4], Π° ΡΠ°ΠΊΠΆΠ΅ ΠΊ ΡΡΠΈΠ»Π΅Π½ΠΈΡ ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΡ ΡΠ²ΠΎΠΉΡΡΠ² ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠΈ [5−9]. ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΡΠΎ, Π² ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ ΡΠΎΡΠΌ ΠΈΠ»ΠΈ Π³ΠΈΠΏΠ΅ΡΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π³Π΅Π½ΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΡ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΡ, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΡΠ΅ ΡΠ°ΠΊΡΠΎΡΡ, ΡΠΈΡΠΎΠ·ΠΈΠ½-ΠΊΠΈΠ½Π°Π·Ρ ΠΈ Π΄ΡΡΠ³ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ.
ΠΡΡΠΈ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ΅ Π²ΠΊΠ»ΡΡΠ°ΡΡ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²ΠΎ Π΄ΡΠ±Π»ΠΈΡΡΡΡΠΈΡ Π΄ΡΡΠ³ Π΄ΡΡΠ³Π° ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², ΠΏΠΎΡΡΠΎΠΌΡ ΠΏΡΠΈ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠ°, ΡΡΠ°ΡΡΠ²ΡΡΡΠ΅Π³ΠΎ Π² ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠ΅ ΡΠΈΠ³Π½Π°Π»Π°, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Π° ΠΊΠΎΠΌΠΏΠ΅Π½ΡΠ°ΡΠΈΡ Π΅Π³ΠΎ ΡΡΠ½ΠΊΡΠΈΠΈ ΠΏΡΡΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΡΠΈ [9]. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ, ΠΏΠΎΠΈΡΠΊ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π΄Π»Ρ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ Π°Π³Π΅Π½ΡΠΎΠ² ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ.
Π ΠΠ-ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΡΠΎΠ±ΠΎΠΉ ΠΏΡΠΎΡΠ΅ΡΡ Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ ΠΌΠ ΠΠ-ΠΌΠΈΡΠ΅Π½ΠΈ, ΠΊΠΎΡΠΎΡΡΠΉ ΠΈΠ½Π΄ΡΡΠΈΡΡΠ΅ΡΡΡ ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π΄Π²ΡΡΠ΅ΠΏΠΎΡΠ΅ΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΡ Π ΠΠ Π΄Π»ΠΈΠ½ΠΎΠΉ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 21−23 Π·Π²Π΅Π½ΡΠ΅Π², Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΡΡΠ°ΡΡΠΊΡ Π΄Π°Π½Π½ΠΎΠΉ ΠΌΠ ΠΠ[10]. ΠΡΠΎΡ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΡΠΏΠΎΡΠΎΠ±ΠΎΠΌ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Π² ΡΡΠΊΠ°ΡΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ , ΠΏΠΎΡΠ²ΠΈΠ²ΡΠΈΠΌΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠ²ΠΎΠ»ΡΡΠΈΠΈ.
ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅
Π² ΠΊΠ»Π΅ΡΠΊΡ Π·1Π ΠΠ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΡΠ½ΠΈΠ·ΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ², Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Ρ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅ΠΌ, Π΄ΠΎ ΡΡΠΎΠ²Π½Ρ, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΡΠΎΠΉ ΠΌΠ ΠΠ Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ . ΠΠ° ΡΠ΅Π³ΠΎΠ΄Π½ΡΡΠ½ΠΈΠΉ Π΄Π΅Π½Ρ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΠ΅ Π ΠΠ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΊΠ°ΠΊ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ, Π½ΠΎ ΠΈ ΠΊΠ°ΠΊ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ Π΄Π»Ρ ΠΏΠΎΠΈΡΠΊΠ° ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π΄Π»Ρ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ, ΡΡΠΎ Π² ΠΊΠ°ΠΆΠ΄ΠΎΠΌ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΠΎΠΌ ΡΠ»ΡΡΠ°Π΅ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΡΠΈΡΠ»Π° Π³Π΅Π½ΠΎΠ², Π°ΠΊΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π° ΠΈΠ»ΠΈ ΡΠ΅ΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½Π° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ, ΠΈ, ΠΈΡΡ ΠΎΠ΄Ρ ΠΈΠ· ΡΡΠΎΠ³ΠΎ, ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ Ρ ΡΠΎΠ½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠΎΠ±ΡΡΠΈΠΉ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ°ΡΠΈΡΠ½ΡΠΌ [9]. Π’Π΅ΠΌ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅, ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΊΠΎΠ΄ΠΈΡΡΡΡ Π±Π΅Π»ΠΊΠΈ, ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠ΅ Π½Π° ΠΏΠ΅ΡΠ΅ΡΠ΅ΡΠ΅Π½ΠΈΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΠΊΠ°ΡΠΊΠ°Π΄ΠΎΠ² ΠΈΠ»ΠΈ Π² Π½Π°ΡΠ°Π»Π΅ ΡΡΠΈΡ ΠΊΠ°ΡΠΊΠ°Π΄ΠΎΠ², ΠΌΠΎΠΆΠ΅Ρ ΠΏΡΠΈΠ²Π΅ΡΡΠΈ ΠΊ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π½Π°Π΄ Π΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΊΠ»Π΅ΡΠΎΠΊ [11]. ΠΠ·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π³Π΅Π½ΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π΅Π·Π° ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΡΡΡΡΡ, Π½ΠΎ ΡΠΎΠ»Ρ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ Π³Π΅Π½Π° Π² ΡΠ»ΠΎΠΆΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅ Ρ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ ΠΎΠ΄Π½ΠΈΠΌ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠ΅Π±ΡΠ΅Ρ ΡΠ³Π»ΡΠ±Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ [12]. ΠΠ°ΡΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π΅ΡΡΡ ΠΊΠ°ΠΊ ΠΊΠ»ΡΡΠ΅Π²ΠΎΠΉ ΡΠ°ΠΊΡΠΎΡ Π² ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΈΠΏΠΎΠ² ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°[9, Π, 12], ΡΠ΅ΠΌ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅, ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ°ΠΊΠΈΡ Π³Π΅Π½ΠΎΠ² Π² ΠΎΠΏΡΡ ΠΎΠ»ΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΠΌΠΎΠΆΠ΅Ρ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΎΡΠ»ΠΈΡΠ°ΡΡΡΡ, ΠΏΠΎΡΡΠΎΠΌΡ Π΄Π»Ρ Π²ΡΠ±ΠΎΡΠ° Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ ΡΡΠ΅Π±ΡΠ΅ΡΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈΠ³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π½Π° ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ.
Π¦Π΅Π»Ρ ΠΈ Π·Π°Π΄Π°ΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°: ΠΠ΅Π³2 (Ρ-Π΅Π³Π¬-Π2, ΠΏΠ΅ΠΉ), Π‘Π‘ΠΠ1 (ΡΠΈΠΊΠ»ΠΈΠ½ Π1) ΠΈ Π ΠΠ‘ (ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΊΠΈΠ½Π°Π·Π° Π‘), Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄ΡΠ ΠΠ ΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ ΡΡΠΈΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. Π Ρ ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΡΠ°Π»ΠΈΡΡ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ Π·Π°Π΄Π°ΡΠΈ:
1. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅Π³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΌΠ°Π»ΡΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΡ Π ΠΠ ΡΠ ΠΠ), Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ Π½Π° ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² ΠΠ΅Π³2, Π‘Π‘Π¨1 ΠΈ Π ΠΠ‘ Π² ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ.
2. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π΄Π»ΠΈΠ½Π½ΡΡ Π΄ΡΠ ΠΠ, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Ρ-ΠΡΡ ΠΈ ΠΠ Π Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.
3. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π½ΡΡΡΡ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π·1Π ΠΠ, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ Π½Π° ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² ΠΠ΅Π³2, Π‘Π‘Π«Π1 ΠΈ Π ΠΠ‘, ΠΏΠΎΡΠ»Π΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ²-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ.
4. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ Π·1Π ΠΠ Π½Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π³Π΅Π½Π½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π² ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΠΈΡ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ.
Π²ΡΠ²ΠΎΠ΄Ρ.
1. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ siPHK (siHer, siCyc, siPKC), Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ ΠΊ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Her2, CCNB1 ΠΈ Π ΠΠ‘. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π΄Π°Π½Π½ΡΠ΅ siPHK ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ²-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΠ-3−1, SK-N-MC, MCF-7 ΠΈ HL-60: Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅Π΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΠΌΠ ΠΠ-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ (Π΄ΠΎ 10 — 20% ΠΎΡ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ) Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΡΠ΅ΡΠ΅Π· 3 ΡΡΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ, Π° ΠΈΡΡ ΠΎΠ΄Π½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π²ΠΎΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°Π΅ΡΡΡ ΡΠ΅ΡΠ΅Π· 7 ΡΡΡΠΎΠΊ.
2. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Her2, CCNB1 ΠΈ Π ΠΠ‘ Ρ ΡΠ°Π·Π½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π·Π°ΠΌΠ΅Π΄Π»ΡΠ΅Ρ Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ-3−1, SK-N-MC, MCF-7, ΠΎΠ΄Π½Π°ΠΊΠΎ Π½Π΅ Π²Π»ΠΈΡΠ΅Ρ Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ HL-60. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΉ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΉ ΡΡΡΠ΅ΠΊΡ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π½Π΅ΠΉΡΠΎΠ±Π»Π°ΡΡΠΎΠΌΡ SK-N-MC ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ siCyc, Π° ΡΠΎΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ MCF-7 Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΡΠΎΡΠΌΠΎΠ·ΠΈΡΡΡ ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ siPKC. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ siPHK, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ ΠΊ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Her2, CCNB1 ΠΈ Π ΠΠ‘, Π½Π° ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ Π²ΠΏΠ΅ΡΠ²ΡΠ΅.
3. ΠΠΎΠ»ΡΡΠ΅Π½Ρ Π΄ΡΠ ΠΠ, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠ΅ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Ρ-ΠΡΡ (dsMyc) ΠΈ GFP (dsEGFP) Π΄Π»ΠΈΠ½ΠΎΠΉ 473 ΠΈ 448 ΠΏ.Π½., ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ dsMyc, Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΠΉ ΠΊΠ°ΠΊ ΠΏΠΎ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ Π ΠΠ-ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΠΈ, ΡΠ°ΠΊ ΠΈ ΡΠ΅ΡΠ΅Π· ΠΈΠ½Π΄ΡΠΊΡΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°, ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½-ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π° Ρ-ΠΡΡ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ, ΡΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ dsEGFP, Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΠΉ ΡΠΎΠ»ΡΠΊΠΎ ΠΏΠΎ ΠΏΡΡΠΈ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠ±Π° ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° Π΄ΡΠ ΠΠ ΡΠΎ ΡΡΠ°Π²Π½ΠΈΠΌΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π·Π°ΠΌΠ΅Π΄Π»ΡΡΡ Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ-3−1 ΠΈ SK-N-MC.
4. ΠΠΏΠ΅ΡΠ²ΡΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΎ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Her2, CCNB1 ΠΈ Π ΠΠ‘. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠΊΠΎΡΠΎΡΡΡ Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ-3−1 Π²ΠΎΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°Π΅ΡΡΡ ΠΏΠΎΡΠ»Π΅ Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ ΠΈΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ²-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΡΠΊΠΎΡΠΎΡΡΡ Π΄Π΅Π»Π΅Π½ΠΈΡ SK-N-MC ΠΎΡΡΠ°Π΅ΡΡΡ Π² 3 — 10 ΡΠ°Π· ΡΠ½ΠΈΠΆΠ΅Π½Π½ΠΎΠΉ Π΄ΠΎ 12 ΡΡΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ siHer, siCyc ΠΈ siPKC, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»ΠΈΠ½Π½ΡΡ Π΄ΡΠ ΠΠ.
5. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π² ΠΊΠ»Π΅ΡΠΊΠΈ siPHK, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ ΠΊ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Her2, CCNB1 ΠΈ Π ΠΠ‘ Π½Π΅ Π²ΡΠ·ΡΠ²Π°Π΅Ρ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ, Π° ΠΏΠΎΠ΄Π°Π²Π»ΡΠ΅Ρ ΠΈΡ Π΄Π΅Π»Π΅Π½ΠΈΠ΅, ΡΡΠΎ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π΅ΡΡΡ Π΄Π°Π½Π½ΡΠΌΠΈ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΠ°ΠΏΡΠΎΡΠΈΠ², Π΄ΠΎΠ»Ρ ΠΌΠ΅ΡΡΠ²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ, ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π΄Π»ΠΈΠ½Π½ΠΎΠΉ Π΄ΡΠ ΠΠ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π΅ΡΡΡ, ΡΡΠΎ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠ΅ΠΉ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° ΠΈ Π²ΡΠ·Π²Π°Π½Π½ΠΎΠΉ ΠΈΠΌ Π³ΠΈΠ±Π΅Π»ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ.
6. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΠΎΠ»Π½ΠΎΠ³Π΅Π½ΠΎΠΌΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΎΠΌΠ° ΠΊΠ»Π΅ΡΠΎΠΊ SK-N-MC, ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ siCyc ΠΈ siPKC, ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ²-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ, ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΡ Π³Π΅Π½ΠΎΠ² (EN02, AK3L1, ALDOC, TXNIP, Π NIP, DDIT4 ΠΈ Π΄Ρ.) ΠΎΡΡΠ°Π΅ΡΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΠΎΠΉ, ΡΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΡΠ»Π°Π²Π»ΠΈΠ²Π°ΡΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ Π°Π½ΡΠΈΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ siPHK Π² ΡΡΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ.
3.10.
ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅
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ΠΠ°ΡΡΡΠ΅Π½ΠΈΡ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°, ΡΠ²Π»ΡΡΡΠΈΠ΅ΡΡ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Π²ΡΠ·Π²Π°Π½Ρ Π³ΠΈΠΏΠ΅ΡΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ ΠΈΠ»ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ Ρ ΠΈΠΌΠ΅ΡΠ½ΡΡ ΠΈΠ»ΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΎΡΠ½ΠΎΡΡΡΡΡ ΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΠΊΠ»Π°ΡΡΠ°ΠΌ Π±Π΅Π»ΠΊΠΎΠ². ΠΠΎΡΡΠΎΠΌΡ ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΡΠΌ ΠΏΠΎΠ΄Ρ ΠΎΠ΄ΠΎΠΌ Π΄Π»Ρ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΡ ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΡ Π ΠΠ, Π²ΡΠ·ΡΠ²Π°ΡΡΠΈΡ Π΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΡ ΠΌΠ ΠΠ-ΠΌΠΈΡΠ΅Π½ΠΈ ΠΏΠΎ ΠΌΠ΅Ρ Π°Π½ΠΈΠ·ΠΌΡ Π ΠΠ-ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΠΈ. Π’Π°ΠΊΠΈΠ΅ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΠ΅ Π ΠΠ ΠΌΠΎΠ³ΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ, ΠΊΠ°ΠΊ ΠΏΡΠΎΡΠΎΠΏΠΈΡΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΡ, Π½ΠΎ ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡΡΡ Π΄Π»Ρ ΠΏΠΎΠΈΡΠΊΠ° Π½ΠΎΠ²ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π΄Π»Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π΄ΡΡΠ³ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. Π ΡΠ΄ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π·1Π ΠΠ ΡΠΆΠ΅ ΠΏΡΠΎΡ ΠΎΠ΄ΠΈΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΠΏΡΡΠ°Π½ΠΈΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ Π²ΡΡΠ²ΠΈΡΡ ΠΊΠ°ΠΊ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π», ΡΠ°ΠΊ ΠΈ ΠΈΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠ΅ ΠΏΠΎΠ±ΠΎΡΠ½ΡΠ΅ ΡΡΡΠ΅ΠΊΡΡ. ΠΠΎΡΠΊΠΎΠ»ΡΠΊΡ ΡΠ΅ΠΏΡ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ΅ Π½ΠΎΡΠΈΡ ΠΊΠ°ΡΠΊΠ°Π΄Π½ΡΠΉ Ρ Π°ΡΠ°ΠΊΡΠ΅Ρ ΠΈ ΡΠΎΡΡΠΎΠΈΡ ΠΈΠ· ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π° Π΄ΡΠ±Π»ΠΈΡΡΡΡΠΈΡ Π΄ΡΡΠ³ Π΄ΡΡΠ³Π° ΠΏΡΡΠ΅ΠΉ, ΡΠΎ Π²Π°ΠΆΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠΈΡΠΊ ΡΠ°ΠΊΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ, ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΊΠΎΡΠΎΡΡΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ Π±Ρ ΠΊ Π½Π΅ΠΎΠ±ΡΠ°ΡΠΈΠΌΡΠΌ ΠΈ Π½Π΅ΠΊΠΎΠΌΠΏΠ΅Π½ΡΠΈΡΡΠ΅ΠΌΡΠΌ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΠ²ΠΈΡΠΌ Π΄Π»Ρ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠΈ, ΠΊ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠ΅ Π΅Π΅ Π΄Π΅Π»Π΅Π½ΠΈΡ.
Π Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΠ΅ Π½Π°ΠΌΠΈ Π±ΡΠ»Π° ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ΠΈΠ° ΠΈ ΠΎΠΏΡΠΎΠ±ΠΈΡΠΎΠ²Π°Π½Π° ΡΡ Π΅ΠΌΠ° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΡ Π ΠΠ Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π²Π³Π ΠΠ, Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² ΠΠ΅Π³2, Π‘Π‘Π«Π1 ΠΈ Π ΠΠ‘, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»ΠΈΠ½Π½ΡΡ Π΄ΡΠ ΠΠ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² Ρ-ΠΡΡ ΠΈ ΠΠ Π . ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π΄Π°Π½Π½ΡΠ΅ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΈΡΡΡΡΠΈΠ΅ Π ΠΠ Ρ ΡΠ°Π·Π½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π·Π°ΠΌΠ΅Π΄Π»ΡΡΡ Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, ΡΡΠΎ ΡΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π½Π° Π½Π΅ΠΎΠ±Ρ ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π΄Π»Ρ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π’ΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ Π΄Π»Ρ Π³Π΅Π½-Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ², ΠΏΡΠ΅Π΄Π½Π°Π·Π½Π°ΡΠ΅Π½Π½ΡΡ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π΅Π½ ΠΠ΅Π³2, ΠΎΠ΄Π½Π°ΠΊΠΎ Π½Π°ΠΌΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ Π΄Π»Ρ Π±Π»ΠΎΠΊΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΎΠΏΡΡ ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΠ‘Π -7 ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π΅Π½ Π ΠΠ‘. ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΌΠΈΡΠ΅Π½ΡΠΌΠΈ Π΄Π»Ρ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΡ ΡΠΎΡΡΠ° Π½Π΅ΠΉΡΠΎΠ±Π»Π°ΡΡΠΎΠΌΡ ΠΠ-Π-ΠΠ‘ ΡΠ²Π»ΡΡΡΡΡ Π³Π΅Π½Ρ Π‘Π‘ΠΠ1 ΠΈ Π ΠΠ‘, ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² Π²ΡΠ·ΡΠ²Π°Π΅Ρ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ, ΠΊΠΎΡΠΎΡΠΎΠ΅ ΡΠΎΡ ΡΠ°Π½ΡΠ΅ΡΡΡ Π΄Π°ΠΆΠ΅ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΈΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ²-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ. ΠΠ°Π½Π½ΡΠ΅ Π³Π΅Π½Ρ ΠΌΠΎΠ³ΡΡ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΌΠΈΡΠ΅Π½ΠΈ Π΄Π»Ρ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π½Π΅ΠΉΡΠΎΠ±Π»Π°ΡΡΠΎΠΌ ΠΈ ΡΠ°ΠΊΠ° ΠΌΠ°Π»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ.
ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π½Π°ΠΌΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² ΠΠ΅Π³2, Π‘Π‘Π«Π1 ΠΈ Π ΠΠ‘ ΠΌΠΎΠ³ΡΡ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ ΠΏΡΠΎΡΠΎΡΠΈΠΏΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΠ΅Π΄ΡΡΠ², Π΄Π»Ρ ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°Π½ΠΈΡ ΡΠΎΡΡΠ° ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΏΠ΅ΡΠ΅ΠΆΠΈΠ²ΡΠΈΡ Ρ ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΡ, ΠΈ, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΌΠΎΠ³ΡΡ ΡΡΠ°ΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΡΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΡ ΠΈ Π½Π΅ΠΉΡΠΎΠ±Π»Π°ΡΡΠΎΠΌΠ°Ρ , Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ. ΠΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΌΠΈΡΠ΅Π½ΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ ΠΏΠΎΠΈΡΠΊΠ° Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΡΡΠ½ΠΊΡΠΈΠΉ ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ², Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΠ΅Π΄ΡΡΠ² Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΡ. ΠΠΏΡΠΎΠ±ΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ Π½Π°ΠΌΠΈ ΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΏΠ»Π°ΡΡΠΎΡΠΌΠ° ΠΌΠΎΠΆΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡΡΡ ΠΏΡΠΈ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠΈΡΠ΅Π½Π΅ΠΉ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΈ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° Π΄Π»Ρ ΡΠ°ΡΡΠΈΡΠ΅Π½Π½ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΠ΅Π»Π΅Π²ΠΎΠΉ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΠΈ.
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