ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π΄ΠΎΠΌΠ΅Π½ΠΎΠ² Π±Π΅Π»ΠΊΠ° Mod (mdg4) Π½Π° ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ Β«ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅ΡΒ» Π² ΡΠ΄ΡΠ°Ρ ΠΊΠ»Π΅ΡΠΎΠΊ Drosophila melanogaster
Drosophila melanogaster2. 1. 2. Π‘ΡΠ΅Π΄Ρ Π΄Π»Ρ Π²ΡΡΠ°ΡΠΈΠ²Π°Π½ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Drosophila melanogaster. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠ΅ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ. 16. ΠΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΡ. Π’Π΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ ΡΠΎΡΠΌ Π±Π΅Π»ΠΊΠ° Mod in vivo. 8. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ. ΠΠ΅ΡΠΎΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅. 5. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ ΡΠ½Π΄ΠΎΠ½ΡΠΊΠ»Π΅Π°Π·Π°ΠΌΠΈ ΡΠ΅ΡΡΡΠΈΠΊΡΠΈΠΈ. Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ. ΠΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΡΡΠΌΠΎΠ»ΠΈΡΡΡΡΡΡ in vitro ΠΈ in vivo… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠ»Π°Π²Π° I. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. Π ΠΠΠ£ΠΠ―Π¦ΠΠ― Π’Π ΠΠΠ‘ΠΠ ΠΠΠ¦ΠΠ. ΠΠΠ‘Π£ΠΠ―Π’ΠΠ Π«
- 1. 1. ΠΠ΅Π½ΠΎΠΌ ΡΡΠΊΠ°ΡΠΈΠΎΡ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½ Π² Π΄ΠΎΠΌΠ΅Π½Ρ
- 1. 2. «ΠΠΎΠ»ΡΠ°ΡΠΈΠ΅» Π΄ΠΎΠΌΠ΅Π½Ρ
- 1. 3. ΠΠΊΡΠΈΠ²Π½ΡΠ΅ Π΄ΠΎΠΌΠ΅Π½Ρ
- 1. 4. ΠΡΠ°Π½ΠΈΡΡ Π΄ΠΎΠΌΠ΅Π½ΠΎΠ²
- 1. 5. ΠΠ½ΡΡΠ»ΡΡΠΎΡΡ
- 1. 5. 1. ΠΠ½ΡΡΠ»ΡΡΠΎΡ Su (Hw)
- 1. 5. 2. ΠΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΠ΅ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΡ Su (Hw) ΠΈ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΡΠ΅Π»ΡΡΠ°
- 1. 5. 3. ΠΠ΅ΠΉΡΡΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠΎΠ²
- 1. 5. 4. ΠΠΎΠ΄Π΅Π»ΠΈ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ° Su (Hw)
- 1. 5. 5. ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² Su (Hw) ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ° Π±Π΅Π»ΠΊΠΎΠΌ SUMO
- 1. Π ΠΠΠ£ΠΠ―Π¦ΠΠ― Π’Π ΠΠΠ‘ΠΠ ΠΠΠ¦ΠΠ. ΠΠΠ‘Π£ΠΠ―Π’ΠΠ Π«
- 2. SUMO: ΠΠΠΠ«Π Π£Π§ΠΠ‘Π’ΠΠΠ ΠΠΠ‘Π’Π’Π ΠΠΠ‘ΠΠ―Π¦ΠΠΠΠΠΠ ΠΠΠΠΠ€ΠΠΠΠ¦ΠΠ
- 2. 1. ΠΡΡΡ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° SUMO
- 2. 2. Π‘ΡΡΠΎΠ΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ° SUMO ΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ²-ΡΡΠ°ΡΡΠ½ΠΈΠΊΠΎΠ² ΠΏΡΡΠΈ ΡΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ
- 2. 2. 1. Π€Π΅ΡΠΌΠ΅Π½Ρ El
- 2. 2. 2. Π€Π΅ΡΠΌΠ΅Π½Ρ Π
- 2. 2. 3. Π€Π΅ΡΠΌΠ΅Π½Ρ ΠΠ
- 2. 3. ΠΠ΅ΡΠΎΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 2. 4. Π‘ΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠ² ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΈ
- 2. 5. Π‘ΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ², Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Ρ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ΅ΠΉ ΠΠΠ
- 2. 6. ΠΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΡΡΠΌΠΎΠ»ΠΈΡΡΡΡΡΡ in vitro ΠΈ in vivo
- 2. 7. ΠΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ±ΠΈΠΊΠ²ΠΈΡΠΈΠ½-Π·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΈ SUMO
- 2. 8. Π€ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΄Π΅ΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ PML
- 2. 1. ΠΠΠ’ΠΠ ΠΠΠΠ«
- 2. 1. 1. Π¨ΡΠ°ΠΌΠΌΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ Π»ΠΈΠ½ΠΈΡ ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 1. 2. Π‘ΡΠ΅Π΄Ρ Π΄Π»Ρ Π²ΡΡΠ°ΡΠΈΠ²Π°Π½ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ ΠΈ ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Drosophila melanogaster
- 2. 1. 3. Π€Π΅ΡΠΌΠ΅Π½ΡΡ ΠΈ ΡΠ΅Π°ΠΊΡΠΈΠ²Ρ
- 2. 1. 4. ΠΠ»ΠΈΠ³ΠΎΠ½ΡΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠ΅ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ
- 2. 2. ΠΠΠ’ΠΠΠ« 57 2.2.1. ΠΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ
- 2. 2. 1. 1. ΠΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π΄Π»Ρ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ
- 2. 2. 1. 2. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π°ΠΌΠΈ
- 2. 2. 1. 3. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ΅Π»ΠΎΡΠ½ΠΎΠ³ΠΎ Π»ΠΈΠ·ΠΈΡΠ°
- 2. 2. 1. 4. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ
- 2. 2. 1. 5. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ ΡΠ½Π΄ΠΎΠ½ΡΠΊΠ»Π΅Π°Π·Π°ΠΌΠΈ ΡΠ΅ΡΡΡΠΈΠΊΡΠΈΠΈ
- 2. 2. 1. 6. ΠΠ³Π°ΡΠΎΠ·Π½ΡΠΉ Π³Π΅Π»Ρ-ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·
- 2. 2. 1. 7. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² ΠΠΠ ΠΈΠ· Π³Π΅Π»Ρ ΠΈ ΠΎΡΠΈΡΡΠΊΠ° ΠΠΠ ΠΎΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΡ ΡΠ΅Π°ΠΊΡΠΈΠΉ
- 2. 2. 1. 8. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ
- 2. 2. 1. 9. ΠΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π· Π² ΠΏΠΎΠ»ΠΈΠ°ΠΊΡΠΈΠ»Π°ΠΌΠΈΠ΄Π½ΠΎΠΌ Π³Π΅Π»Π΅
- 2. 2. 1. 10. ΠΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π΄ΡΠΎΠΆΠΆΠ΅ΠΉ
- 2. 2. 1. 11. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 2. 1. 12. ΠΠ½Π°Π»ΠΈΠ· Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² Π΄Π²ΡΠ³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅
- 2. 2. 1. 13. Π’ΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ S2 ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 2. 1. 14. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ Π»ΠΈΠ·Π°ΡΠ° ΠΈΠ· S2 ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 2. 1. 15. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΄Π΅ΡΠ½ΠΎΠ³ΠΎ Π»ΠΈΠ·Π°ΡΠ° ΠΈΠ· S2 ΠΊΠ»Π΅ΡΠΎΠΊ
- 2. 2. 1. 16. ΠΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΡ
- 2. 2. 1. 17. ΠΠΌΠΌΡΠ½ΠΎΠΏΡΠ΅ΡΠΈΠΏΠΈΡΠ°ΡΠΈΡ Ρ ΡΠΎΠΌΠ°ΡΠΈΠ½Π°
- 2. 2. 1. 18. Western-Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ
- 2. 2. 1. 18. ΠΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ²
- 2. 2. 1. 19. Π ΠΠ-ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅ΡΠΈΡ
- 2. 2. 1. 20. ΠΠ΅ΡΠ΅ΠΊΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² Π½Π° ΠΏΠΎΠ»ΠΈΡΠ΅Π½Π½ΡΡ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ°Ρ Π΄ΡΠΎΠ·ΠΎΡΠΈΠ»Ρ
- 2. 2. 1. 21. ΠΠ΅ΡΠ΅ΠΊΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΈΠΌΠ°Π³ΠΈΠ½Π°Π»ΡΠ½ΡΡ Π΄ΠΈΡΠΊΠΎΠ² Π΄ΡΠΎΠ·ΠΎΡΠΈΠ»Ρ
- 2. 2. 1. 22. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ, ΡΠΊΡΠΏΡΡΡΡΠΈΡΡΡΠΈΡ Π±Π΅Π»ΠΊΠΈ CP 190, Mod (mdg4)-67.2 ΠΈ Π΅Π³ΠΎ Π²Π°ΡΠΈΠ°Π½ΡΡ Π² 82-ΠΊΠ»Π΅ΡΠΊΠ°Ρ Drosophila
- 2. 2. 1. 23. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Π΄Π»Ρ Π΄ΡΠΎΠΆΠΆΠ΅Π²ΠΎΠΉ Π΄Π²ΡΠ³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ
- 2. 2. 1. 24. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Π΄Π»Ρ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π² Π»ΠΈΡΠΈΠ½ΠΊΠΈ ΠΌΡΡ 72 2.2.1. ΠΠ΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ
- 2. 2. 1. ΠΠΈΠ½ΠΈΠΈ Drosophila melanogaster, ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π½ΡΠ΅ Π² ΡΠ°Π±ΠΎΡΠ΅
- 2. 2. 2. Π€Π΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ²
- 2. 2. 3. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΡΠΌΠ±ΡΠΈΠΎΠ½ΠΎΠ² Drosophila melanogaster ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠ°Π½ΡΠ³Π΅Π½Π½ΡΡ Π»ΠΈΠ½ΠΈΠΉ
- 1. ΠΡΡΠ°Π½ΡΠ½ΡΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΡ Π±Π΅Π»ΠΊΠ° Mod (mdg4), ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠ΅ Π² ΡΠ°Π±ΠΎΡΠ΅
- 2. ΠΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Mod (mdg4) ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ Π² 82-ΠΊΠ»ΡΡΠΊΠ°Ρ
- 3. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² Π±Π΅Π»ΠΊΠ° Mod Π² ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΡΠ΄Π΅ΡΠ½ΠΎΠΉ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΡΡΠ°ΠΊΡΠΈΡΡ S2 ΠΊΠ»Π΅ΡΠΎΠΊ
- X. -ChIP Π°Π½Π°Π»ΠΈΠ·
- 4. Π’Π΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ ΡΠΎΡΠΌ Π±Π΅Π»ΠΊΠ° Mod in vivo
- 5. ΠΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΏΡΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ Π±Π΅Π»ΠΊΠ° Mod (mdg4) Π² Π»ΠΈΡΠΈΠ½ΠΊΠ°Ρ 86 Π§ΠΠ‘Π’Π¬ II. ΠΠ«Π―ΠΠΠΠΠΠ Π€ΠΠΠ’ΠΠ Π, ΠΠ’ΠΠΠ’Π‘Π’ΠΠΠΠΠΠΠ ΠΠ ΠΠΠ ΠΠΠΠΠΠΠΠ «ΠΠΠ‘Π£ΠΠ―Π’ΠΠ ΠΠ«Π₯ Π’ΠΠΠΠ¦» ΠΠΠΠΠ MOD (MDG4)
- 1. Π£Π΄Π°Π»Π΅Π½ΠΈΠ΅ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² ΡΠ΄Π΅ΡΠ½ΠΎΠΉ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° Mod (mdg4) Π²Π»ΠΈΡΠ΅Ρ Π½Π° ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ° Π² ΠΊΠ»Π΅ΡΠΊΠ΅ ΠΈ Π½Π΅ Π²Π»ΠΈΡΠ΅Ρ Π½Π° Π΅Π³ΠΎ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π°
- 2. ΠΡΠΎΡΠ΅ΡΡ ΡΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ³ΡΠ°Π΅Ρ ΠΊΠ»ΡΡΠ΅Π²ΡΡ ΡΠΎΠ»Ρ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ ΡΠΏΠ΅ΠΊΠ»ΠΎΠ² Π±Π΅Π»ΠΊΠ° Mod (mdg4)
- 3. Π£Π½ΠΈΠΊΠ°Π»ΡΠ½ΡΠ΅ Π‘-ΠΊΠΎΠ½ΡΠ΅Π²ΡΠ΅ Π΄ΠΎΠΌΠ΅Π½Ρ Π±Π΅Π»ΠΊΠ° Mod (mdg4) Π½Π΅ Π½ΡΠΆΠ½Ρ Π΄Π»Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ»
- 4. ΠΠ΅ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π±Π΅Π»ΠΎΠΊ Su (Hw) ΡΠΏΠΎΡΠΎΠ±Π΅Π½ ΠΎΠ±ΡΠ°Π·ΠΎΠ²ΡΠ²Π°ΡΡ ΡΠΏΠ΅ΠΊΠ»Ρ
- 5. ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠΏΠ΅ΠΊΠ»ΠΎΠΎΠ±ΡΠ°Π·ΡΡΡΠ΅Π³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π΄ΠΎΠΌΠ΅Π½ΠΎΠ² Π±Π΅Π»ΠΊΠ° Mod (mdg4) Π½Π° ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ Β«ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅ΡΒ» Π² ΡΠ΄ΡΠ°Ρ ΠΊΠ»Π΅ΡΠΎΠΊ Drosophila melanogaster (ΡΠ΅ΡΠ΅ΡΠ°Ρ, ΠΊΡΡΡΠΎΠ²Π°Ρ, Π΄ΠΈΠΏΠ»ΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ)
ΠΠΊΠΎΠ»ΠΎ Π΄Π΅ΡΡΡΠΈ Π»Π΅Ρ Π½Π°Π·Π°Π΄ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π±Π΅Π»ΠΊΠΈ Su (Hw) ΠΈ Mod (mdg4) ΠΊΠΎΠ»ΠΎΠΊΠ°Π»ΠΈΠ·ΡΡΡΡΡ Π² Π΄ΠΈΡΠΊΡΠ΅ΡΠ½ΡΡ ΡΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡΡ Π² ΡΠ΄ΡΠ°Ρ ΠΈΠ½ΡΠ΅ΡΡΠ°Π·ΠΈΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Drosophila. ΠΡΠ½ΠΎΠ²ΡΠ²Π°ΡΡΡ Π½Π° ΡΠΎΠΌ, ΡΡΠΎ ΠΈΡΡΠ΅Π·Π½ΠΎΠ²Π΅Π½ΠΈΠ΅ ΡΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠΈΡΡΡΡΠΈΡ ΡΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠΉ ΠΏΡΠΈ ΠΌΡΡΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° Mod (mdg4) ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΎΠ²Π°Π»ΠΎ ΠΎΡΠ»Π°Π±Π»Π΅Π½ΠΈΡ Su (Hw) ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ°, ΡΠ°ΠΊΠΈΠ΅ ΡΠ΄Π΅ΡΠ½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ Π½Π°Π·Π²Π°Π½Ρ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠΌΠΈ ΡΠ΅Π»ΡΡΠ°ΠΌΠΈ».
ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΡΡΡ, ΡΡΠΎ ΡΡΠΈ ΡΠ΅Π»ΡΡΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ Π·Π°ΡΠΈΠΊΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π½Π° ΡΠ΄Π΅ΡΠ½ΠΎΠΌ ΠΌΠ°ΡΡΠΈΠΊΡΠ΅ ΡΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ ΠΌΠ½ΠΎΠ³ΠΈΡ ΡΠ°Π·Π½Π΅ΡΠ΅Π½Π½ΡΡ ΠΏΠΎ Π³Π΅Π½ΠΎΠΌΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² ΠΠΠ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ° Su (Hw). ΠΠΎ ΠΊΠ°ΠΊΠΎΠΉ-ΡΠΎ ΠΏΡΠΈΡΠΈΠ½Π΅ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΡ ΠΎΠ±ΡΠ΅Π΄ΠΈΠ½ΡΡΡΡΡ Π΄ΡΡΠ³ Ρ Π΄ΡΡΠ³ΠΎΠΌ ΠΈ Π΄Π΅ΡΠΆΠ°ΡΡΡ Π²ΠΌΠ΅ΡΡΠ΅ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ Mod (mdg4) ΠΈ CP 190, ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ ΠΎΠ±ΡΠ°Π·ΡΡ «ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΠ΅ Ρ ΡΠΎΠΌΠ°ΡΠΈΠ½ΠΎΠ²ΡΠ΅ ΠΏΠ΅ΡΠ»ΠΈ-Π΄ΠΎΠΌΠ΅Π½Ρ» ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡ Π²ΡΡΠΎΠΊΠΎΡΠΏΠΎΡΡΠ΄ΠΎΡΠ΅Π½Π½ΡΡ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ°. ΠΡΠ½ΠΎΠ²ΡΠ²Π°ΡΡΡ Π½Π° ΡΡΠΎΠΌ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ, Π±ΡΠ»Π° Π²ΡΠ΄Π²ΠΈΠ½ΡΡΠ° ΡΡΡΡΠΊΡΡΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ°, ΠΏΠΎΡΡΡΠ»ΠΈΡΡΡΡΠ°Ρ, ΡΡΠΎ ΡΠ½Ρ Π°Π½ΡΠ΅Ρ, Π½Π°Ρ ΠΎΠ΄ΡΡΠΈΠΉΡΡ Π² ΠΎΠ΄Π½ΠΎΠΌ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΌ Π΄ΠΎΠΌΠ΅Π½Π΅, Π½Π΅ ΠΌΠΎΠΆΠ΅Ρ Π°ΠΊΡΠΈΠ²ΠΈΡΠΎΠ²Π°ΡΡ ΠΏΡΠΎΠΌΠΎΡΠΎΡ, Π½Π°Ρ ΠΎΠ΄ΡΡΠΈΠΉΡΡ Π² Π΄ΡΡΠ³ΠΎΠΌ Π΄ΠΎΠΌΠ΅Π½Π΅.
Π ΡΠΎΠΆΠ°Π»Π΅Π½ΠΈΡ, ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΠΎΠ΅ ΡΠΎΡΡΠ΅Π΄ΠΎΡΠΎΡΠ΅Π½ΠΈΠ΅ ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠΎΠ² Π²Π½ΡΡΡΠΈ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ» Π½Π΅ Π±ΡΠ»ΠΎ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΎ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΌΠ½ΠΎΠ³ΠΈΡ Π»Π΅Ρ. ΠΠΎΡΡΠΎΠΌΡ Π² ΡΠ°Π±ΠΎΡΠ΅ Π±ΡΠ»ΠΈ ΠΏΠΎΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ Π·Π°Π΄Π°ΡΠΈ:
1. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ²ΡΠ·Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ 8ΠΈ (ΠΡ)-Π·Π°Π²ΠΈΡΠΈΠΌΡΡ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠΎΠ² ΠΈ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π²Π½ΡΡΡΠΈΡΠ΄Π΅ΡΠ½ΡΡ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ».
2. ΠΠ·ΡΡΠΈΡΡ ΡΠΎΠ»Ρ ΡΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ».
3. ΠΠΎΠ½ΡΡΡ ΡΠΎΠ»Ρ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ».
Π²ΡΠ²ΠΎΠ΄Ρ.
ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ ΠΏΡΡΠΌΠΎΠΉ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π²Π½ΡΡΡΠΈΡΠ΄Π΅ΡΠ½ΡΡ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ» ΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ 8ΠΈ (ΠΠ»Ρ)-Π·Π°Π²ΠΈΡΠΈΠΌΡΡ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠΎΠ² Ρ ΠΠ³ΠΎ. ΡΠΎΡΠΠΠ° ΡΠ΅1Π°ΠΏΠΎ%Π°$Π₯Π΅Π³.
ΠΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° ΠΠΎΡ1(ΡΡ4) Π² ΡΠΎΡΡΠ°Π² «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ» ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π΅Π³ΠΎ ΡΡΠΌΠΎΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ, ΡΡΠΎ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΠΉ Π±Π΅Π»ΠΎΠΊ Π‘Π 190 ΠΈΠ³ΡΠ°Π΅Ρ ΠΊΠ»ΡΡΠ΅Π²ΡΡ ΡΠΎΠ»Ρ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ».
ΠΡΠ΄Π²ΠΈΠ½ΡΡΠ° Π³ΠΈΠΏΠΎΡΠ΅Π·Π° ΠΎ ΡΠΎΠ»ΠΈ «ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠ΅Π»Π΅Ρ» ΠΊΠ°ΠΊ «ΡΠ°Π±ΡΠΈΠΊΠΈ» ΠΏΠΎ Π±ΡΡΡΡΠΎΠΉ ΡΠ±ΠΎΡΠΊΠ΅ ΠΈΠ½ΡΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΠΈ Ρ ΡΠ°Π½Π΅Π½ΠΈΡ Π΅Π³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ².
Π‘ΠΏΠΈΡΠΎΠΊ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
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